Discovery VP Preparative System
The Discovery VP preparative system enables purification and collection of milligram to gram quantities of compounds generated from Drug Discovery, Medicinal Chemistry, Natural Product Isolation, and Organic Synthesis efforts. Multiple fraction collectors increase throughput and facilitate high volume purification of lead candidates. The system is totally automated from injection to detection, collection, and report generation. User-friendly software makes operation easy for chromatographers of all experience levels. A simple interface, pictorial view of sample location, and Administrator suggested run conditions let chemists submit samples with only a few mouse clicks. Fraction collection is triggered by a UV detector, mass spectrometer, or an 'other' detector such as RID or ELSD. An identical interface to the Discovery VP Analytical System enables the two systems to work side by side to first establish analysis conditions, then scale up for purification of synthesis products.
Milligram to Gram Quantities of Compounds
The Discovery VP Preparative system can accommodate up to six columns of any size. Typical sample load for a 20 mm diameter column is up to 300 milligrams, and 50 mm diameter columns can handle 2 grams of material. Follow individual column manufacturer's recommendations for sample loading and injection volumes.
Multiple Fraction Collectors
The multiplexing technology of Discovery VP lets you configure up to six fraction collectors for more than 800 5mL or 350 30 mL fractions. This scalability is crucial for facilities with a large number of users that generate many compounds daily. Software options let you choose the collection volume to isolate one peak per tube, or break up a large peak and save only the most highly concentrated fractions.
Totally Automated
Discovery VP's full automation eliminates the need for an operator to continually monitor the hardware and prepare the column for each injection. At startup, Discovery VP can prime the pumps, and before each injection the column is flushed and equilibrated and a baseline check is done to ensure stability. When all samples are complete, Discovery VP will flush all of the columns used in the queue and turn the power off to the pumps and detector.
Report Generation
Users have a choice of two report formats in the Preparative system. One is a chromatogram and area percent report generated by CLASS-VP with a separate fraction collector report, and the other is a one-page 'integrated' report that consists of a chromatogram with peak shading to indicate filled tubes, and the tube location in one of up to six fraction collectors.
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Pictorial View of Sample Location
Discovery VP supports several sample rack types for maximum flexibility. The Administrator configures the system for 100 2 mL vials, 80 4 mL vials, 25 13 mL vials, or two 96 or 384-position microtiter plates. click for picture
Run Conditions set by the Administrator
The Administrator sets all of the column parameters such as maximum pressure and flow rate allowed, and can designate a 'default' method for each column so that when it is selected by a user, run conditions are automatically loaded. The default method contains frequently used run conditions and saves the user time during sample submission.
Fraction Collection Discovery VP collects fractions based on slope and level signal values coming from the detector. The Administrator establishes a range of values and users can adjust the sensitivity of the fraction collector to control the number of fractions obtained per run. When multiple detectors are configured, collection can be triggered by the UV or 'other' detector (MS, RID, or ELSD) signal.
Enabling Technology for the Pharmaceutical Industry
Enabling Technology for the Pharmaceutical Industry
Discovery VP Automated Systems
Discovery VP Analytical System
